Naoqing formula helps protect the brain from stroke damage
Author Information
Author(s): Ye Yujun, Xie Xuexin, Bi Yiming, Liu Qing, Weng Xuliang, Qiu Lingling, Zhao He, Hei Shangyan, Yang Ling, Wang Chengyin, Zhu Weifeng, Zeng Ting
Primary Institution: The Affiliated Traditional Chinese Medicine Hospital, Guangzhou Medical University
Hypothesis
Naoqing formula alleviates acute ischaemic stroke through the modulation of Nrf2-mediated ferroptosis.
Conclusion
Naoqing formula protects against acute ischaemic stroke by reducing ferroptosis and oxidative stress, primarily through the activation of the Nrf2 pathway.
Supporting Evidence
- Naoqing formula increased cortical blood flow and reduced neuronal damage in stroke models.
- Transcriptomic and proteomic analyses indicated that Naoqing formula regulates lipid and iron metabolism through the Nrf2 pathway.
- Nrf2, xCT, and GPX4 levels were upregulated in Naoqing formula-treated mice.
- Naoqing formula reduced oxidative stress and iron overload in stroke models.
- Inhibition of Nrf2 expression aggravated ferroptosis and reduced neuroprotection from Naoqing formula.
Takeaway
This study shows that a traditional Chinese medicine called Naoqing formula can help protect the brain from damage caused by strokes by stopping a type of cell death called ferroptosis.
Methodology
Mice and SH-SY5Y cells were used to study the effects of Naoqing formula on stroke-induced ferroptosis, employing molecular biological techniques, transcriptomics, and proteomics.
Limitations
The study does not fully elucidate the molecular mechanisms by which Naoqing formula affects acute ischaemic stroke.
Participant Demographics
Male C57BL/6 mice were used in the study.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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