Using Multicellular Tumour Spheroids to Monitor Breast Cancer Treatment
Author Information
Author(s): Monazzam Azita, Razifar Pasha, Simonsson Martin, Qvarnström Fredrik, Josephsson Raymond, Blomqvist Carl, Långström Bengt, Bergström Mats
Primary Institution: Uppsala University
Hypothesis
Can [18F]FDG be used as a biomarker for monitoring early response to chemotherapy in breast cancer using multicellular tumour spheroids?
Conclusion
The study demonstrated that multicellular tumour spheroids are a suitable model for evaluating the potential of FDG-PET in early breast cancer treatment monitoring.
Supporting Evidence
- Paclitaxel and Docetaxel significantly reduced [18F]FDG uptake in BT474 spheroids.
- Doxorubicin reduced [18F]FDG uptake more in MCF-7 than in BT474 spheroids.
- Tamoxifen only reduced [18F]FDG uptake in MCF-7 spheroids at the highest dose.
- No effect of Imatinib was observed on [18F]FDG uptake.
Takeaway
Researchers used special cell clusters to see how well a cancer imaging technique works for checking if breast cancer treatments are effective.
Methodology
The study involved treating multicellular tumour spheroids with various chemotherapy agents and measuring [18F]FDG uptake and viable volume after treatment.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website