ESTuber Database for Tuber borchii Sequences
Author Information
Author(s): Lazzari Barbara, Caprera Andrea, Cosentino Cristian, Stella Alessandra, Milanesi Luciano, Viotti Angelo
Primary Institution: Istituto di Biologia e Biotecnologia Agraria
Hypothesis
The aim was to produce an extensive and easily accessible database for truffle, providing sequence analysis and annotation, as well as links to related resources.
Conclusion
The resulting web site is a resource of data and links related to truffle expressed genes.
Supporting Evidence
- The ESTuber db includes 3,271 Tuber borchii expressed sequence tags (EST).
- The dataset consists of sequences from both in-house prepared cDNA libraries and downloaded sequences from GenBank.
- An automated pipeline was developed to process EST sequences using public software and in-house scripts.
Takeaway
The ESTuber database collects and organizes genetic information about Tuber borchii, a type of truffle, to help researchers study its genes.
Methodology
A cDNA library was prepared from in vitro cultured mycelia, and extensive EST sequencing was performed, followed by clustering and annotation against multiple databases.
Limitations
The limited number of sequences does not allow for significant analysis at the moment.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website