Bone Marrow Infiltration in B-cell Non-Hodgkin's Lymphoma
Author Information
Author(s): E.L. Dorey, S.V. Outram, A. Holder, A.Z.S. Rohatiner, F.E. Cotter, M. Deane, A.G. Stansfeld, T.A. Lister, M.A. Horton
Primary Institution: Imperial Cancer Research Fund, St Bartholomew's Hospital
Hypothesis
The study aims to establish the normal range of CD20 positive lymphocytes in bone marrow and compare levels in patients with NHL before and after therapy.
Conclusion
The study found that CD20 positive lymphocytes were significantly higher in morphologically involved marrow compared to normal subjects.
Supporting Evidence
- CD20 positive lymphocytes were significantly higher in morphologically involved marrow than in normal subjects.
- The mean percentage of CD20 positive cells in uninvolved bone marrow was consistent before and after therapy.
- Patients with NHL had a greater range of CD20 positive cells compared to normal subjects.
Takeaway
Doctors looked at bone marrow from people with lymphoma and found more cancer cells than expected, which helps understand how to treat the disease better.
Methodology
Bone marrow samples were taken from patients and normal subjects, and the levels of CD20 positive lymphocytes were measured using flow cytometry.
Limitations
The study may not detect very small populations of neoplastic cells due to the limitations of immunophenotyping.
Participant Demographics
20 normal subjects (12 men, 8 women, age 19-43) and 85 patients with NHL (49 men, 36 women, age 12-80).
Statistical Information
P-Value
p<0.00001
Statistical Significance
p<0.00001
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