Degrading Levofloxacin with Fungal Secretome
Author Information
Author(s): Staita Karima, Khmaissa Marwa, Akrout Imen, Greff Stéphane, Ghariani Bouthaina, Turbé-Doan Annick, Lambert Julien, Lomascolo Anne, Albert Quentin, Faulds Craig B., Sciara Giuliano, Zouari-Mechichi Héla, Record Eric, Mechichi Tahar
Primary Institution: Laboratoire de Biochimie et de Génie Enzymatique des Lipases, Ecole Nationale d’Ingénieurs de Sfax, Université de Sfax, Tunisia
Hypothesis
Can the secretome of Coriolopsis gallica effectively degrade the fluoroquinolone antibiotic levofloxacin?
Conclusion
The free secretome of Coriolopsis gallica can degrade levofloxacin more effectively than the immobilized version, achieving up to 100% biotransformation under optimal conditions.
Supporting Evidence
- The free secretome achieved a maximum removal efficiency of 50 mg L−1 levofloxacin.
- The immobilized secretome was able to degrade only 10 mg L−1 levofloxacin under the same conditions.
- Biotransformation rates were influenced by factors such as pH, temperature, and the concentration of the chemical mediator HBT.
Takeaway
Scientists found that a special fungus can break down a common antibiotic in water, which is good for the environment. They tested different conditions to see how well it works.
Methodology
The study involved testing the degradation of levofloxacin by both free and immobilized secretome of Coriolopsis gallica under various physical-chemical conditions.
Limitations
The immobilized secretome showed reduced efficiency compared to the free secretome, possibly due to substrate diffusion limitations.
Digital Object Identifier (DOI)
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