Prolonged and enhanced suppression of thymidylate synthase by weekly 24-h infusion of high-dose 5-fluorouracil
2000
High-Dose 5-Fluorouracil and Thymidylate Synthase Suppression
publication
Evidence: moderate
Author Information
Author(s): Yeh K-H, Yeh S-H, Hsu C-H, Wang T-M, Ma I-F, Cheng A-L
Primary Institution: National Taiwan University Hospital
Hypothesis
Does prolonged exposure to low concentrations of 5-FU lead to better suppression of thymidylate synthase compared to short exposure to high concentrations?
Conclusion
Prolonged exposure to low concentrations of 5-FU results in better suppression of thymidylate synthase and enhanced cytotoxicity compared to short exposure to high concentrations.
Supporting Evidence
- 24-h exposure to low concentration of 5-FU resulted in better suppression of free TS.
- Higher degree of S-phase blockade was observed with prolonged exposure.
- Enhanced cytotoxicity was noted with the 24-h regimen compared to the 30-min regimen.
Takeaway
Giving cancer cells a little bit of medicine for a long time works better than giving them a lot of medicine for a short time.
Methodology
In vitro studies using human gastric cancer cell line NCI-N87 to compare effects of different 5-FU regimens.
Digital Object Identifier (DOI)
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