Decellularizing Corneal Stroma Using N2 Gas
Author Information
Author(s): Amano Shiro, Shimomura Naoki, Yokoo Seiichi, Araki-Sasaki Kaoru, Yamagami Satoru
Primary Institution: University of Tokyo School of Medicine
Hypothesis
To examine the efficacy of a novel method of decellularizing porcine corneal stroma using N2 gas and its feasibility in a corneal transplantation model in rabbits.
Conclusion
The method using N2 gas decellularizes corneal stroma without reducing corneal transparency.
Supporting Evidence
- The decellularized porcine corneal stroma remained clear for six months after transplantation into rabbits.
- Histological examination showed few keratocytes infiltrated the decellularized porcine cornea six months after transplantation.
- Significantly fewer stained cellular nuclei were observed in the corneal stroma of the N2 group than in the control groups.
Takeaway
This study shows a new way to remove cells from pig corneas using nitrogen gas, which keeps the corneas clear and ready for transplanting into rabbits.
Methodology
Porcine corneas were treated with N2 gas to induce decellularization, followed by histological examination and transplantation into rabbits.
Limitations
The long-term effects of the decellularized cornea on transparency and keratocyte function need further investigation.
Participant Demographics
Porcine corneas from 12 fresh pig eyes obtained from a slaughterhouse.
Statistical Information
P-Value
p=0.0054
Statistical Significance
p<0.0001
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