Purinergic receptors and tooth-pulp pain
Author Information
Author(s): Adachi Kazunori, Shimizu Kohei, Hu James W, Suzuki Ikuko, Sakagami Hiroshi, Koshikawa Noriaki, Sessle Barry J, Shinoda Masamichi, Miyamoto Makiko, Honda Kuniya, Iwata Koichi
Primary Institution: Nihon University School of Dentistry
Hypothesis
Are P2X receptors involved in responses to noxious pulp stimulation?
Conclusion
Activation of P2X3 and P2X2/3 receptors in the tooth pulp elicits nociceptive behavioral responses and trigeminal brainstem neuronal activity.
Supporting Evidence
- GG muscle activity increased significantly after pulpal application of 100 mM α,β-meATP compared to PBS.
- pERK-LI cell expression was significantly higher in the Vc and C1/C2 regions after α,β-meATP application.
- Co-application of TNP-ATP reduced both GG activity and pERK-LI cell expression.
- Intrathecal injection of PD 98059 significantly reduced pERK-LI cell expression and GG activity.
Takeaway
When certain chemicals are applied to the tooth pulp, they can cause pain responses in the brain and muscles, showing how tooth pain works.
Methodology
The study used 75 male Sprague-Dawley rats to analyze the effects of P2X receptor agonists on muscle activity and neuronal responses following tooth pulp stimulation.
Limitations
The insertion of a paper point into the tooth pulp may itself cause strong mechanical stimulation, which could confound results.
Participant Demographics
75 male Sprague-Dawley adult rats (300-360 g)
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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