Lignolytic Enzymes from Mushroom Stereum ostrea
Author Information
Author(s): K. Praveen, Viswanath B., Usha K. Y., Pallavi H., Venkata Subba Reddy G., Naveen M., Rajasekhar Reddy B.
Primary Institution: Sri Krishnadevaraya University
Hypothesis
The study investigates the production of lignolytic enzymes by the mushroom Stereum ostrea compared to the reference culture Phanerochaete chrysosporium.
Conclusion
Stereum ostrea produces a complete lignolytic system and is more effective in laccase production than Phanerochaete chrysosporium.
Supporting Evidence
- Stereum ostrea produced 25 units of laccase/ml, significantly higher than the 8.9 units/ml from Phanerochaete chrysosporium.
- Stereum ostrea also showed higher activities of lignin peroxidase and manganese peroxidase compared to the reference culture.
- The study confirmed the presence of lignolytic enzymes through the decolorisation of the dye Remazol orange 16.
Takeaway
The mushroom Stereum ostrea can break down tough plant materials better than another fungus, making it useful for cleaning up the environment.
Methodology
The study involved growing Stereum ostrea and Phanerochaete chrysosporium in a liquid medium and measuring the production of lignolytic enzymes over time.
Limitations
The study only examined enzyme production under noninducing conditions.
Digital Object Identifier (DOI)
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