The Role of Period 2 Gene in Breast Cancer
Author Information
Author(s): Xiang Shulin, Coffelt Seth B, Mao Lulu, Yuan Lin, Cheng Qi, Hill Steven M
Primary Institution: Tulane University Health Sciences Center
Hypothesis
The clock gene, Per 2, is expressed in normal human mammary epithelium and at a reduced level in breast cancer cells leading to an alteration in the cell cycle, cell growth, and cell survival.
Conclusion
Expression of PER 2 in MCF-7 breast cancer cells significantly inhibits cell growth and induces apoptosis.
Supporting Evidence
- PER 2 expression significantly inhibited the growth of MCF-7 breast cancer cells.
- Co-expression of PER 2 and Cry 2 resulted in an even greater growth-inhibitory effect.
- Expression of PER 2 induced apoptosis in MCF-7 cells as evidenced by increased PARP cleavage.
Takeaway
The study found that a gene called PER 2 helps stop breast cancer cells from growing and can even make them die, which is important for fighting cancer.
Methodology
The study used immunoblot analyses, cell proliferation assays, soft agar clonogenic assays, and cell cycle studies to assess the effects of PER 2 on breast cancer cells.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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