Donor variability in HIV binding to peripheral blood mononuclear cells
2008
Variability in HIV Binding to Blood Cells
Sample size: 19
publication
Evidence: moderate
Author Information
Author(s): Joshua J Anzinger, Gene G Olinger, Gregory T Spear
Primary Institution: National Heart, Lung, and Blood Institute, National Institutes of Health
Hypothesis
HIV attachment variability exists between donor cells.
Conclusion
Differences exist in HIV binding to donor PBMC, which is CD4-independent and can change over time.
Supporting Evidence
- HIV binding to PBMC varied up to 3.9-fold between individuals.
- Binding phenotypes were maintained over a 4-week period for most donors.
- HIV binding variability was independent of CD4 and virus strain.
Takeaway
Different people have different levels of HIV binding to their blood cells, and this can change over time.
Methodology
HIV binding was assessed using freshly isolated PBMC from 19 healthy donors and measured by ELISA.
Limitations
The study does not identify the specific mechanisms behind the variability in HIV binding.
Participant Demographics
Healthy donors.
Digital Object Identifier (DOI)
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