Discovery of New Splice Variants in Pig PPARGC1A
Author Information
Author(s): Tim Erkens, Karel Bilek, Alex Van Zeveren, Luc J Peelman
Primary Institution: Ghent University
Hypothesis
How the functions of PPARGC1A are regulated in pigs is largely unclear.
Conclusion
The study identified two new splice variants of the PPARGC1A gene in pigs, which likely affect the protein's function.
Supporting Evidence
- Two new splice variants were detected in porcine tissues.
- Both splice variants were also found in human liver and one in human kidney.
- The splice variants could lead to shorter proteins that may alter the gene's function.
Takeaway
Scientists found two new versions of a gene in pigs that might change how the gene works, which is important for understanding meat quality.
Methodology
Tissue samples were collected from pigs, RNA was extracted, and cDNA was synthesized to identify splice variants using exon-spanning primers.
Limitations
The functional analysis of the splice variants was not performed, limiting the understanding of their effects.
Participant Demographics
Samples were taken from a freshly slaughtered female commercial hybrid pig and a male pig.
Digital Object Identifier (DOI)
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