Integrated Linkage Map of the Silkworm Genome
Author Information
Author(s): Yamamoto Kimiko, Nohata Junko, Kadono-Okuda Keiko, Narukawa Junko, Sasanuma Motoe, Sasanuma Shun-ichi, Minami Hiroshi, Shimomura Michihiko, Suetsugu Yoshitaka, Banno Yutaka, Osoegawa Kazutoyo, de Jong Pieter J, Goldsmith Marian R, Mita Kazuei
Primary Institution: National Institute of Agrobiological Sciences
Hypothesis
To conduct a complete genome analysis of the silkworm Bombyx mori by integrating genetic and physical maps.
Conclusion
The integrated map contains approximately 10% of predicted silkworm genes and has an estimated 76% genome coverage by BACs.
Supporting Evidence
- 1,755 SNP markers were mapped onto 28 linkage groups.
- 6,221 contigs were constructed from BAC libraries.
- Approximately 361.1 Mb of BAC contigs were integrated with the genetic map.
Takeaway
Scientists created a detailed map of the silkworm's DNA to help understand its genes better.
Methodology
The study involved mapping SNP markers onto linkage groups and constructing BAC contigs using fingerprinting methods.
Limitations
The genome remains somewhat fragmented due to shallow coverage from previous sequencing methods.
Participant Demographics
The study used inbred silkworm strains p50T and C108T.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website