Proteomic Analysis of Skin Invasion by Schistosome Larvae
Author Information
Author(s): Hansell Elizabeth, Braschi Simon, Medzihradszky Katalin F., Sajid Mohammed, Debnath Moumita, Ingram Jessica, Lim K. C., McKerrow James H.
Primary Institution: University of California, San Francisco
Hypothesis
What proteins are released by schistosome cercariae during their invasion of human skin?
Conclusion
The study identified proteins released by schistosome larvae during skin invasion and highlighted the host's biochemical defenses against this invasion.
Supporting Evidence
- Proteins released by cercariae include enzymes that degrade host tissue.
- Host proteins such as immunoglobulins and complement factors were identified in the skin.
- Apoptosis of epidermal cells was documented during cercarial invasion.
- Specific degradation of host proteins was observed in skin invaded by cercariae.
Takeaway
When tiny schistosome larvae invade human skin, they release proteins that help them get in, while the skin tries to fight back with its own proteins.
Methodology
Human skin samples were exposed to cercariae for 30 minutes to 2 hours, and proteins were analyzed using LC/MS/MS.
Potential Biases
Potential bias due to the use of a single human skin sample from a surgical amputation.
Limitations
The study used non-perfused human skin, which may not fully represent the in vivo environment.
Participant Demographics
Human skin from a patient with peripheral vascular disease.
Digital Object Identifier (DOI)
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