Human oesophageal adenocarcinoma cell lines JROECL 47 and JROECL 50 are admixtures of the human colon carcinoma cell line HCT 116
2000
Study of Oesophageal Adenocarcinoma Cell Lines
publication
Author Information
Author(s): Wijnhoven B P L, Tilanus M G J, Morris A G, Darnton S J, Tilanus H W, Dinjens W N M
Primary Institution: Erasmus University Medical Centre Rotterdam
Hypothesis
Are the human oesophageal adenocarcinoma cell lines JROECL 47 and JROECL 50 admixtures of the human colon carcinoma cell line HCT 116?
Conclusion
The study found that the cell lines JROECL 47 and JROECL 50 contain identical mutations as the colon carcinoma cell line HCT 116.
Supporting Evidence
- The cell lines JROECL 47 and JROECL 50 were derived from one tumour.
- Identical E-cadherin and β-catenin gene mutations were detected.
Takeaway
Scientists looked at two types of cancer cells and found they were mixed with another type of cancer cell.
Methodology
The study used HLA-typing, mutation analysis, and microsatellite analysis.
Digital Object Identifier (DOI)
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