Enhancing Disease Resistance in Creeping Bentgrass Using Antimicrobial Peptide
Author Information
Author(s): Zhou Man, Hu Qian, Li Zhigang, Chen Dayong, Luo Hong
Primary Institution: Clemson University
Hypothesis
Can the antimicrobial peptide Penaeidin4-1 enhance disease resistance in creeping bentgrass against fungal pathogens?
Conclusion
The study demonstrates that the antimicrobial peptide Pen4-1 effectively enhances resistance to major fungal diseases in creeping bentgrass.
Supporting Evidence
- Transgenic plants showed a 42% to 48% reduction in lesion size from R. solani infection compared to controls.
- Pen4-1 expression was confirmed at the transcription level in all transgenic lines.
- Transgenic lines exhibited high resistance against both dollar spot and brown patch diseases.
Takeaway
Scientists added a special protein from shrimp to grass plants to help them fight off diseases caused by fungi, and it worked really well!
Methodology
Transgenic creeping bentgrass plants were created using Agrobacterium-mediated transformation to express the Penaeidin4-1 peptide, followed by in vitro and in vivo assays to test disease resistance.
Limitations
The study did not successfully detect the Pen4-1 protein in transgenic plants, which may affect the understanding of its functional expression.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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