How Adenovirus Type 5 Affects E-Cadherin Dynamics
Author Information
Author(s): Hussain Fawziyah, Morton Penny E., Snippe Marjolein, Sullivan Janis, Farmer Charlotte, Martin-Fernandez Marisa L., Parsons Maddy, Santis George
Primary Institution: King's College London
Hypothesis
Does intact Adenovirus Type 5 disrupt cell junctions and alter paracellular permeability at an early stage in its infectious cycle?
Conclusion
Adenovirus Type 5 increases paracellular permeability and alters E-cadherin dynamics in MCF7 cells.
Supporting Evidence
- Adenovirus Type 5 fiber protein binds to CAR, disrupting cell junctions.
- Increased paracellular permeability was observed shortly after Ad5 addition.
- High CAR expression reduced E-cadherin levels at cell junctions.
- Ad5eGFP increased the mobility of E-cadherin in MCF7 cells.
- Ad5eGFP did not alter CAR levels or localization.
Takeaway
Adenovirus Type 5 can make it easier for itself to enter cells by breaking down the connections between those cells.
Methodology
The study used MCF7 cells and CAR overexpressing MCF7 cells to assess the effects of Adenovirus Type 5 on E-cadherin dynamics and paracellular permeability.
Limitations
The significance of the findings in naturally occurring Adenovirus Type 5 infections cannot be determined.
Participant Demographics
Human breast cancer cells (MCF7) were used in the study.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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