Purification of human respiratory syncytial virus by ultracentrifugation in iodixanol density gradient
2008
Purification of Human Respiratory Syncytial Virus Using Iodixanol
publication
Evidence: high
Author Information
Author(s): Gias E., Nielsen S.U., Morgan L.A., Toms G.L.
Primary Institution: The University of Newcastle upon Tyne
Hypothesis
Can iodixanol improve the recovery rate of infectious human respiratory syncytial virus (hRSV) particles compared to traditional sucrose gradients?
Conclusion
Iodixanol density gradient centrifugation yields high recovery of purified infectious hRSV particles.
Supporting Evidence
- Iodixanol increased the virus yield up to 69%.
- Iodixanol was found to be non-toxic to HeLa cells used in infectivity assays.
- The recovery rate of the virus ranged from 66 to 71%.
Takeaway
The study found a better way to clean a virus called hRSV using a special liquid that helps keep the virus safe and healthy.
Methodology
The study used ultracentrifugation with iodixanol gradients to purify hRSV from infected cell cultures.
Limitations
The study does not specify the limitations of the methodology used.
Digital Object Identifier (DOI)
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