Spt2p Defines a New Transcription-Dependent Gross Chromosomal Rearrangement Pathway
2008
Spt2p and Its Role in Chromosomal Rearrangements
publication
Evidence: moderate
Author Information
Author(s): Nilabja Sikdar, Soma Banerjee, Han Zhang, Stephanie Smith, Myung Kyungjae
Primary Institution: National Human Genome Research Institute, National Institutes of Health
Hypothesis
Spt2p overexpression could lead to gross chromosomal rearrangements (GCR).
Conclusion
Excess Spt2p enhances GCR formation by increasing single-stranded DNA and disrupting transcription regulation.
Supporting Evidence
- Excess Spt2p expression increased GCR formation up to 1,600 fold.
- High transcription levels in chromosome V enhanced GCR formation.
- Mutations in genes required for de novo telomere addition reduced GCRs caused by excess Spt2p.
Takeaway
When a protein called Spt2p is too high in cells, it can cause problems with DNA that lead to cancer.
Methodology
The study used yeast as a model organism to investigate the effects of Spt2p overexpression on GCR formation.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website