Semaphorin-4D (Sema-4D), the Plexin-B1 ligand, is involved in mouse ovary follicular development
2007
Role of Semaphorin-4D in Mouse Ovary Follicular Development
Sample size: 30
publication
Evidence: moderate
Author Information
Author(s): Regev Avital, Goldman Shlomit, Shalev Eliezer
Primary Institution: Ha'Emek Medical Center and Technion-Israel Institute of Technology
Hypothesis
The effect of Plexin-B1 in the ovarian follicle is mediated by sema-4D.
Conclusion
Semaphorin-4D modulates follicular development rates in the mouse ovary.
Supporting Evidence
- Sema-4D is expressed in the mouse ovary, mainly in granulosa cells.
- In vitro growth of follicles was enhanced in the presence of Sema-4D.
- Neutralizing antibodies against Plexin-B1 abolished the effects of Sema-4D.
Takeaway
This study shows that a protein called Semaphorin-4D helps mouse ovaries grow and develop properly.
Methodology
In vivo expression and localization of Sema-4D were tested by immunohistochemistry, and its role in follicular development was examined using in vitro growth of preantral follicles.
Limitations
The study could not examine endothelial cell formation.
Participant Demographics
Female ICR mice, 12-day-old and 26-day-old.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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