Fluorescent Labelling System for Listeria monocytogenes
Author Information
Author(s): Andersen Jens B, Roldgaard Bent B, Lindner Ariel B, Christensen Bjarke B, Licht Tine R
Primary Institution: Danish Institute for Food and Veterinary Research
Hypothesis
Can a fluorescent marker system be developed to allow for the discrimination of different strains of Listeria monocytogenes in co-invasion studies?
Conclusion
The fluorescent marker system is stable, easy to use, and does not affect the virulence of Listeria monocytogenes in Caco-2 cell assays.
Supporting Evidence
- The fluorescent marker system allows visualization and identification of single L. monocytogenes cells.
- More than 90% of the L. monocytogenes host cells maintained the fluorescence tags for 40 generations.
- The fluorescence tags did not alter the invasive capacity of the L. monocytogenes cells in a traditional Caco-2 cell invasion assay.
Takeaway
Researchers created a special coloring system that helps them see different types of Listeria bacteria without hurting them, making it easier to study how they invade cells.
Methodology
A multicolour tagging system was developed using various fluorescent proteins and tested in Caco-2 cell invasion assays.
Limitations
The study does not provide full information about infection at the levels of complex tissues and individuals.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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