Choosing Reference Genes for PCR in Boechera Plants
Author Information
Author(s): Pellino Marco, Sharbel Timothy F, Mau Martin, Amiteye Samuel, Corral José María
Primary Institution: Leibniz-Institute für Pflanzengenetik und Kulturpflanzenforschung (IPK)
Hypothesis
De-regulation of the sexual developmental pathway could trigger apomictic reproduction.
Conclusion
The study identified BoechRPS18, BoechEfα1, BoechACT2, and BoechUBQ as the most stable reference genes for gene expression normalization in apomictic and sexual Boechera.
Supporting Evidence
- The study tested six reference genes for stability in gene expression.
- BoechRPS18 and BoechEfα1 were identified as the most stable genes for apomictic plants.
- Different optimal combinations of reference genes were found for various tissue types.
Takeaway
Scientists found the best genes to use as a reference for measuring other genes in certain plants, which helps in understanding how these plants reproduce.
Methodology
The study evaluated six potential reference genes using qRT-PCR on RNA from various reproductive and vegetative tissues of Boechera.
Limitations
The study focused only on specific tissues and may not generalize to all plant types or conditions.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website