Reducing Pseudomonas fluorescens Biofilm with Phage and Chlorine
Author Information
Author(s): Mendes Matheus B., Vidigal Pedro M. P., Soto Lopez Maryoris E., Hungaro Humberto M.
Primary Institution: Universidade Federal de Juiz de Fora (UFJF)
Hypothesis
Can the combination of the UFJF_PfSW6 phage and sodium hypochlorite effectively reduce Pseudomonas fluorescens biofilms?
Conclusion
The UFJF_PfSW6 phage combined with sodium hypochlorite significantly reduces Pseudomonas fluorescens biofilms in the food industry.
Supporting Evidence
- The UFJF_PfSW6 phage reduced bacterial counts by 1.3 to 2.0 logs CFU/cm2 depending on conditions.
- Sequential application of phage and sodium hypochlorite achieved greater reductions in biofilm counts.
- Phage treatment was more effective against young biofilms compared to mature ones.
Takeaway
This study shows that using a special virus and chlorine together can help clean bacteria that stick to surfaces in food production.
Methodology
The study compared the effects of two lytic phages and sodium hypochlorite on biofilms formed by Pseudomonas fluorescens on stainless steel at different temperatures and times.
Limitations
The study did not achieve complete eradication of biofilms.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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