Origin of marrow stromal cells and haemopoietic chimaerism following bone marrow transplantation determined by in situ hybridisation
1990

Origin of Bone Marrow Stromal Cells After Transplantation

Sample size: 7 publication Evidence: moderate

Author Information

Author(s): N.A. Athanasou, J. Quinn, M.K. Brenner, H. Grant Prentice, A. Graham, S. Taylor, D. Flannery, J. O'D. McGee

Primary Institution: Nuffield Department of Pathology, University of Oxford

Hypothesis

Is there a common stem cell for both haemopoietic and stromal cells in bone marrow?

Conclusion

Stromal cells in bone marrow transplants are of host origin, indicating distinct lineages for haemopoietic and stromal cells.

Supporting Evidence

  • Stromal cells were identified as being of host origin in all examined cases.
  • Mixed chimaerism was observed in haemopoietic cells, indicating donor and host contributions.
  • The study used a novel technique to examine intact bone marrow without cell dissociation.

Takeaway

When people get bone marrow transplants, the new cells that help make blood come from the donor, but the support cells in the bone marrow come from the person who received the transplant.

Methodology

In situ hybridisation was used to detect the Y chromosome in bone marrow sections from patients who received transplants from donors of the opposite sex.

Limitations

The study had a small sample size and may not represent all cases of bone marrow transplantation.

Participant Demographics

Seven patients (four female, three male) aged 10-43, treated for acute leukaemia.

Want to read the original?

Access the complete publication on the publisher's website

View Original Publication