Vaccinia Virus and APOBEC3 Proteins
Author Information
Author(s): Melanie Kremer, Yasemin Suezer, Yolanda Martinez-Fernandez, Carsten Münk, Gerd Sutter, Barbara S Schnierle
Primary Institution: Paul-Ehrlich-Institut
Hypothesis
Is vaccinia virus replication affected by APOBEC3G and other family members?
Conclusion
APOBEC3G is not a restriction factor for vaccinia virus replication, and the virus does not degrade APOBEC3G.
Supporting Evidence
- APOBEC3G expression did not inhibit vaccinia virus replication in HeLa cells.
- Viral titers in APOBEC3G-expressing cells were slightly higher than in non-expressing cells.
- Other members of the APOBEC3 family also did not affect vaccinia virus replication.
Takeaway
This study found that a protein called APOBEC3G, which helps fight some viruses, does not stop the vaccinia virus from making copies of itself.
Methodology
HeLa and 293T cells were transfected with APOBEC3G and infected with vaccinia virus to measure viral replication.
Limitations
The lack of a positive control to show that APOBEC3 still confers an inhibitory function on retroviruses during VACV infection.
Digital Object Identifier (DOI)
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