Claudin-1 is a Target Gene of p63 in Skin Development
Author Information
Author(s): Lopardo Teresa, Lo Iacono Nadia, Marinari Barbara, Giustizieri Maria L., Cyr Daniel G., Merlo Giorgio, Crosti Francesca, Costanzo Antonio, Guerrini Luisa
Primary Institution: Department of Biomolecular Sciences and Biotechnology, University of Milan, Milan, Italy
Hypothesis
p63 regulates Claudin-1 expression, linking it to skin development and ectodermal dysplasias.
Conclusion
Claudin-1 is an important target gene of p63, essential for normal skin development and barrier function.
Supporting Evidence
- Claudin-1 expression is significantly reduced when p63 is silenced in keratinocytes.
- Claudin-1 is absent in the skin of p63 null mice.
- Mutations in p63 associated with AEC syndrome impair its ability to regulate Claudin-1 transcription.
- Claudin-1 expression is crucial for maintaining skin barrier function.
Takeaway
This study shows that a gene called Claudin-1 is important for skin health and is controlled by another gene called p63.
Methodology
The study involved experiments with primary mouse keratinocytes, siRNA-mediated gene silencing, and chromatin immunoprecipitation to analyze gene expression.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website