Long-term stability of ASFV vaccine candidate ASFV-G-ΔI177L
Author Information
Author(s): Espinoza Nallely, Spinard Edward, Rai Ayushi, Ramirez-Medina Elizabeth, Valladares Alyssa, Meyers Amanda, Borca Manuel V., Gladue Douglas P.
Primary Institution: Plum Island Animal Disease Center, Agricultural Research Service, USDA
Hypothesis
Can lyophilization improve the stability of the ASFV-G-ΔI177L vaccine for long-term storage?
Conclusion
The ASFV-G-ΔI177L vaccine remains stable for up to one year at 4 °C when lyophilized under specific conditions.
Supporting Evidence
- Lyophilization allows the vaccine to be stored without ultra-cold conditions.
- The vaccine maintained similar titers after storage.
- Next-generation sequencing confirmed the genome remained unchanged after lyophilization.
Takeaway
Scientists found a way to keep a pig vaccine stable for a whole year without needing super cold storage, which is really important for places that don't have it.
Methodology
The study evaluated different lyophilization conditions and stabilizer formulations for the ASFV-G-ΔI177L vaccine to determine its stability during storage.
Limitations
Further studies are needed to optimize storage conditions for higher temperatures.
Digital Object Identifier (DOI)
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