Enhancing Digestive Enzyme Activity in Enterococcus faecalis
Author Information
Author(s): Yuan Meng, Li Zhengzhong, Zhou Qunlan, Zheng Xiaochuan, Sun Cunxin, Liu Bo, Wang Aimin, Zhu Aimin
Primary Institution: Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences
Hypothesis
Can ARTP mutagenesis increase the digestive enzyme activity of Enterococcus faecalis?
Conclusion
Three mutant strains of Enterococcus faecalis were developed that significantly increased digestive enzyme production and demonstrated stable inheritance.
Supporting Evidence
- 833 mutant strains were obtained after ARTP treatment.
- EF-448, EF-798, and EF-804 showed a 180% increase in α-amylase activity.
- The strains exhibited high auto-aggregation capacity and cell hydrophobicity.
- Survival rates of EF-448 and EF-798 in simulated intestinal fluid were 85.71% and 82.32%, respectively.
- The strains retained antioxidant capacity and showed no hemolysis.
- No mortality was observed in Macrobrachium rosenbergii after injections of mutant strains.
Takeaway
Scientists used a special method to change some bacteria so they could help digest food better, making them useful for animals and humans.
Methodology
E. faecalis was treated with ARTP mutagenesis, and the resulting mutant strains were screened for digestive enzyme activity.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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