Characterization of a New Tomato Leaf Curl Virus Variant Affecting Eggplant in India
Author Information
Author(s): Pratap Dharmendra, Kashikar Ashwin R, Mukherjee Sunil K
Primary Institution: International Centre for Genetic Engineering and Biotechnology
Hypothesis
Is there a begomovirus variant causing yellow mosaic disease in eggplant in India?
Conclusion
The study identifies a new variant of Tomato leaf curl New Delhi virus that infects eggplant and causes yellow mosaic disease.
Supporting Evidence
- The virus variant was confirmed to cause yellow mosaic symptoms in eggplants and leaf curling in tomatoes.
- The study is the first to demonstrate Koch's postulate for a begomovirus associated with eggplant yellow mosaic disease.
- The complete DNA-A and DNA-B genomic components of the virus were cloned and sequenced.
Takeaway
Scientists found a new virus that makes eggplants sick, causing yellow spots on their leaves.
Methodology
The researchers used genomic DNA isolation, rolling circle amplification, cloning, and agroinfiltration techniques to study the virus.
Limitations
The study did not detect the presence of a DNA-β satellite molecule associated with the virus.
Participant Demographics
Infected leaf samples were collected from various locations in Nagpur, Central India.
Digital Object Identifier (DOI)
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