Dramatic Transcriptional Changes in an Intracellular Parasite Enable Host Switching between Plant and Insect
2011

Phytoplasmas Change Gene Expression When Switching Hosts

Sample size: 6 publication Evidence: moderate

Author Information

Author(s): Kenro Oshima, Yoshiko Ishii, Shigeyuki Kakizawa, Kyoko Sugawara, Yutaro Neriya, Misako Himeno, Nami Minato, Chihiro Miura, Takuya Shiraishi, Yasuyuki Yamaji, Shigetou Namba

Primary Institution: The University of Tokyo

Hypothesis

How phytoplasmas can adapt to two diverse environments is of considerable interest.

Conclusion

Phytoplasmas dramatically alter their gene expression in response to host switching between plant and insect hosts.

Supporting Evidence

  • Approximately 33% of the genes change upon host switching.
  • The expression of the gene encoding the secreted protein PAM486 was highly upregulated in the plant host.
  • Phytoplasma growth in planta was partially suppressed by an inhibitor of the MscL osmotic channel.

Takeaway

Phytoplasmas are tiny bacteria that can live in both plants and insects, and they change how they behave depending on which one they are in.

Methodology

Microarray analysis was used to evaluate gene expression profiles of phytoplasmas grown in plant and insect hosts.

Limitations

The study primarily focuses on one strain of phytoplasma and may not represent all phytoplasmas.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0023242

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