Histone Acetyltransferases Sas3p and Gcn5p Bind to Similar Genes
Author Information
Author(s): Rosaleny Lorena E, Ruiz-García Ana B, García-Martínez José, Pérez-Ortín José E, Tordera Vicente
Primary Institution: Departament de Bioquímica i Biologia Molecular, Universitat de València, València, Spain
Hypothesis
Do the histone acetyltransferases Sas3p and Gcn5p bind to similar sets of genes in yeast?
Conclusion
Sas3p and Gcn5p are recruited to a similar pool of intensely transcribed genes, suggesting they act as general activators.
Supporting Evidence
- Sas3p and Gcn5p show considerable overlap in the genes they bind.
- Both proteins positively correlate with the acetylation state of Lys14 of histone H3.
- The study provides evidence that Sas3p acts as a transcriptional activator, similar to Gcn5p.
Takeaway
This study found that two proteins, Sas3p and Gcn5p, help turn on similar genes in yeast by adding a special tag to a part of the DNA called histone H3.
Methodology
A macroarray platform was used to identify binding sites of yeast histone acetyltransferases and correlate their positions with acetylation of histone H3.
Limitations
The macroarray method cannot distinguish between binding sites within intergenic regions and those within an ORF.
Statistical Information
P-Value
3.8 × 10-13
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website