Evc2 is a positive modulator of Hedgehog signalling that interacts with Evc at the cilia membrane and is also found in the nucleus
2011
Evc2 and Its Role in Hedgehog Signaling
publication
Evidence: high
Author Information
Author(s): Blair Helen J, Tompson Stuart, Liu Yu-Ning, Campbell Jennifer, MacArthur Katie, Ponting Chris P, Ruiz-Perez Victor L, Goodship Judith A
Primary Institution: Institute of Human Genetics, Newcastle University
Hypothesis
Evc2 is a positive regulator of Hedgehog signaling.
Conclusion
Evc2 is essential for Hedgehog signaling and interacts with Evc at the basal body of primary cilia.
Supporting Evidence
- Evc2 is essential for Hedgehog pathway activation in response to the Smo agonist purmorphamine.
- Evc and Evc2 co-localize at the basal body and cilia membrane.
- Evc2 is found in the nucleus, while Evc is not.
Takeaway
Evc2 helps a signaling pathway that is important for growth and development, and it works together with another protein called Evc.
Methodology
The study involved bioinformatic analysis, yeast two-hybrid screening, immunoprecipitation, and various cell culture experiments.
Digital Object Identifier (DOI)
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