Human Cells Resistant to HIV-1 Infection
Author Information
Author(s): Lech Patrycja, Somia Nikunj V
Primary Institution: University of Minnesota
Hypothesis
Can human cells be isolated that are resistant to HIV-1 infection?
Conclusion
Human cell mutants can be isolated that are resistant to infection by HIV-1, and the resistance is genetically recessive.
Supporting Evidence
- Two mutant cell lines were isolated that exhibit up to 10-fold resistance to HIV-1 infection.
- The resistance phenotype is not due to an entry defect.
- The mutations conferring resistance are recessive.
Takeaway
Scientists found two types of human cells that can resist getting sick from a virus called HIV-1. This means they can help us understand how to stop the virus.
Methodology
HeLa cells were chemically mutagenized and screened for resistance to HIV-1 infection using a viral vector encoding a toxic gene.
Limitations
The study may not have identified all possible resistant clones due to the nature of the screening process.
Participant Demographics
Human HeLa cells were used in the study.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website