High Level Expression and Purification of HIV-1 p24 Protein
Author Information
Author(s): Zhang Baozhong, Liu Dabin, Bao Zuoyi, Chen Bin, Li Cun, Jiang Huanhuan, Wang Xiaona, Mi Zhiqiang, An Xiaoping, Lu Jun, Tong Yigang
Primary Institution: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
Hypothesis
The study aims to express and purify the p24 protein in soluble form in E. coli.
Conclusion
The soluble recombinant p24 protein specifically reacts with HIV infected sera and elicits HIV p24 specific antibodies in mice, indicating its potential as a reagent for HIV diagnosis.
Supporting Evidence
- The recombinant p24 protein was highly expressed in soluble form after induction in E. coli.
- SDS-PAGE analysis showed that the His-tagged recombinant p24 protein was successfully purified.
- Indirect ELISA demonstrated that the recombinant p24 protein specifically reacted with HIV-infected human serum samples.
- The immunogenicity of the p24 protein was confirmed by the production of specific antibodies in immunized mice.
Takeaway
Scientists made a special protein from HIV that can help doctors find out if someone is infected with the virus. They tested it on mice and it worked well.
Methodology
The p24 protein was expressed in E. coli, purified using nickel affinity chromatography, and tested for immunogenicity in mice.
Participant Demographics
The study included 90 human serum samples, with 40 HIV-1 positive and 50 HIV-1 negative samples.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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