Calnexin is not essential for mammalian rod opsin biogenesis
2008
Calnexin is not essential for mammalian rod opsin biogenesis
publication
Evidence: moderate
Author Information
Author(s): Kosmaoglou Maria, Cheetham Michael E.
Primary Institution: UCL Institute of Ophthalmology
Hypothesis
Does calnexin play a critical role in the biogenesis of mammalian rod opsin?
Conclusion
Mammalian rod opsin biogenesis does not require calnexin, unlike Drosophila Rh1.
Supporting Evidence
- Wild-type rod opsin translocated normally to the plasma membrane in both cell lines.
- P23H rod opsin was retained in the ER in both cell lines.
- The only difference observed was a small increase in the incidence of P23H intracellular inclusions in the sCnx cells.
Takeaway
Calnexin is like a helper for proteins, but for mammalian rod opsin, it’s not needed to help it grow properly.
Methodology
Mouse embryonic fibroblasts from wild-type and calnexin-deficient mice were used to study rod opsin processing.
Limitations
The study did not include the retina in the phenotyping of the Cnx null or sCnx mice.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Want to read the original?
Access the complete publication on the publisher's website