Re-Assembly of the Genome of Francisella tularensis Subsp. holarctica OSU18
Author Information
Author(s): Puiu Daniela, Salzberg Steven L., Hahn Matthew W.
Primary Institution: Center for Bioinformatics and Computational Biology, University of Maryland, College Park, Maryland, United States of America
Hypothesis
Can the genome of Francisella tularensis OSU18 be accurately re-assembled to correct mis-assemblies?
Conclusion
The re-assembly of the F. tularensis OSU18 genome corrected two large inversion mis-assemblies, providing a more accurate genome for future studies.
Supporting Evidence
- The original genome assembly contained two large inversion errors.
- The corrected assembly provides a more accurate basis for future comparative studies.
- Detailed information on the placement of individual reads was generated during the re-assembly.
Takeaway
Scientists fixed mistakes in the genetic blueprint of a germ that can make people sick, which helps in studying it better.
Methodology
The genome was re-assembled using de novo and comparative assembly techniques, correcting mis-assemblies identified in the original sequence.
Limitations
Some finishing reads were not available, leading to expected gaps in the assembly.
Digital Object Identifier (DOI)
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