An Antibody to the Lutheran Glycoprotein (Lu) Recognizing the LU4 Blood Type Variant Inhibits Cell Adhesion to Laminin α5
2011
Antibody to Lutheran Glycoprotein Inhibits Cell Adhesion to Laminin α5
publication
Evidence: moderate
Author Information
Author(s): Kikkawa Yamato, Miwa Takahiro, Tohara Yukiko, Hamakubo Takayuki, Nomizu Motoyoshi
Primary Institution: Tokyo University of Pharmacy and Life Sciences
Hypothesis
Can a function-blocking antibody inhibit the binding of Lu/B-CAM to laminin α5?
Conclusion
The function-blocking antibody against Lu/B-CAM can inhibit cell adhesion to laminin α5 and may help develop drugs for sickle cell disease.
Supporting Evidence
- The antibody 87207 inhibited the binding of Lu-Fc to laminin α5.
- Combining antibody 87207 with anti-integrin antibodies significantly reduced cell adhesion.
- Mutagenesis studies indicated that the D2 domain is involved in the binding of laminin α5.
Takeaway
Researchers found an antibody that stops a protein from sticking to a part of the body, which could help treat a blood disease.
Methodology
The study used a binding assay on tissue sections to test the effects of a function-blocking antibody on Lu/B-CAM binding to laminin α5.
Digital Object Identifier (DOI)
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