Raf-1 Activation Prevents Caspase 9 Processing Downstream of Apoptosome Formation
2011
Raf-1 Activation Prevents Caspase 9 Processing in Apoptosis
publication
Evidence: moderate
Author Information
Author(s): Sébastien Cagnol, Anna Mansour, Ellen Van Obberghen-Schilling, Jean-Claude Chambard
Primary Institution: Institute of Developmental Biology and Cancer, CNRS UMR6543, University of Nice Sophia-Antipolis
Hypothesis
Does sustained stimulation of the Raf-1/MAPK1,3 pathway prevent caspase-9 activation induced by serum depletion?
Conclusion
The Raf-1/MAPK1,3 pathway inhibits caspase-9 activation in the apoptosome complex, preventing apoptosis.
Supporting Evidence
- Raf-1 activation prevents caspase-9 cleavage and activity following serum removal.
- MEK inhibitors reversed the protective effects of Raf-1 on caspase-9.
- Protein synthesis is required for Raf-1-mediated inhibition of caspase-9.
Takeaway
When cells are starved of growth factors, they usually die, but activating a specific pathway can stop this from happening.
Methodology
The study used CCL39-ΔRaf-1:ER fibroblasts to investigate the effects of Raf-1 activation on caspase-9 processing during serum deprivation.
Digital Object Identifier (DOI)
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