Butyrate's Effects on Bovine Kidney Cells
Author Information
Author(s): Li Robert W, Li CongJun
Primary Institution: Bovine Functional Genomics Laboratory, Animal and Natural Resources Institute, USDA-ARS
Hypothesis
How does sodium butyrate affect gene expression in bovine kidney epithelial cells?
Conclusion
Butyrate significantly alters gene expression related to cell cycle control and apoptosis in bovine kidney epithelial cells.
Supporting Evidence
- 450 genes were significantly regulated by butyrate with a median False Discovery Rate of 0%.
- Real-time PCR confirmed the microarray results with a strong positive correlation (R = 0.867).
- Butyrate repressed the majority of cell cycle control-related genes.
Takeaway
Butyrate, a substance found in the gut, can change how certain genes work in cow kidney cells, which might help cows grow better and fight off diseases.
Methodology
The study used high-density oligonucleotide microarrays to analyze gene expression in MDBK cells treated with sodium butyrate.
Limitations
The study only examined a single time point and dose due to budget constraints.
Participant Demographics
Bovine kidney epithelial cells (MDBK) were used in the study.
Statistical Information
P-Value
0
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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