Preventing Chromosome Misalignment in Yeast
Author Information
Author(s): Gregan Juraj, Riedel Christian G., Pidoux Alison L., Katou Yuki, Rumpf Cornelia, Schleiffer Alexander, Kearsey Stephen E., Shirahige Katsuhiko, Allshire Robin C., Nasmyth Kim
Primary Institution: Max F. Perutz Laboratories, University of Vienna
Hypothesis
The Pcs1/Mde4 complex is essential for preventing merotelic attachment of kinetochores during mitosis and meiosis II.
Conclusion
The Pcs1/Mde4 complex is crucial for accurate chromosome segregation by preventing merotelic attachment in fission yeast.
Supporting Evidence
- Pcs1 and Mde4 localize to the central core of centromeres.
- Mutations in pcs1+ or mde4+ lead to high frequencies of lagging chromosomes.
- The Pcs1/Mde4 complex is suggested to clamp microtubule binding sites together.
Takeaway
This study shows that two proteins, Pcs1 and Mde4, help ensure that chromosomes are pulled to the right place during cell division, preventing mistakes.
Methodology
The study used tandem affinity purification and mass spectrometry to identify protein complexes and analyzed chromosome behavior through various assays.
Digital Object Identifier (DOI)
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