Rapid Detection of Ofloxacin in Meat Using a Test Strip
Author Information
Author(s): Li Xiaolan, Huang Jin, Li Na, Salah Mahmoud, Guan Shuoning, Pan Wenwen, Wang Ziyi, Zhou Xinghua, Wang Yun
Primary Institution: School of Food and Biological Engineering, Jiangsu University
Hypothesis
Can a colloidal gold immunochromatographic assay strip effectively detect ofloxacin residues in animal-derived foods?
Conclusion
The developed immunological detection method can effectively detect ofloxacin residues in meat products.
Supporting Evidence
- The IC50 of the enzyme-linked immunosorbent assay (ic-ELISA) was 0.13 ng/mL.
- The detection limit was 0.033 ng/mL.
- The visual detection limit of the established test strip was 1 ng/g.
- The recovery rates for pork, fish, and chicken were within the range of 80%~120%.
Takeaway
Scientists created a quick test to check if meat has a harmful antibiotic called ofloxacin in it, which can be bad for our health.
Methodology
The study used monoclonal antibodies to create a test strip for detecting ofloxacin in meat, measuring sensitivity and specificity through various assays.
Digital Object Identifier (DOI)
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