Nasal Anti-CD3 Induces Tr1 Cells in Mice
Author Information
Author(s): Wu Henry Yim, Quintana Francisco J., da Cunha Andre Pires, Dake Benjamin T., Koeglsperger Thomas, Starossom Sarah C., Weiner Howard L.
Primary Institution: Brigham and Women's Hospital, Harvard Medical School
Hypothesis
Can nasal administration of anti-CD3 antibody induce Tr1 cells in vivo?
Conclusion
Nasal administration of anti-CD3 antibody effectively induces suppressive Tr1 cells in mice through a mechanism dependent on IL-27 and AHR signaling.
Supporting Evidence
- Nasal anti-CD3 treatment increased the frequency of IL-10 producing T cells.
- IL-27 produced by dendritic cells was essential for Tr1 cell differentiation.
- AHR signaling was required for the generation of Tr1 cells in response to nasal anti-CD3.
- Tr1 cells induced by nasal anti-CD3 were shown to suppress immune responses.
Takeaway
This study shows that giving a special antibody through the nose can help create cells that calm down the immune system, which is important for treating diseases where the immune system attacks the body.
Methodology
Mice were treated with nasal anti-CD3 antibody, and the effects on Tr1 cell generation were analyzed through flow cytometry and cytokine assays.
Limitations
The study primarily focuses on mouse models, which may not fully translate to human conditions.
Participant Demographics
The study used female mice, specifically MRL/lpr and various transgenic strains.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website