Foxp3 and IL-10 Expression Correlates with Parasite Burden in Lesional Tissues of Post Kala Azar Dermal Leishmaniasis (PKDL) Patients
2011

Foxp3 and IL-10 Levels in Post Kala Azar Dermal Leishmaniasis Patients

Sample size: 25 publication Evidence: high

Author Information

Author(s): Katara Gajendra Kumar, Ansari Nasim Akhtar, Verma Sandeep, Ramesh V., Salotra Poonam

Primary Institution: Institute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi, India

Hypothesis

The study investigates the correlation of natural regulatory T cell markers and IL-10 with parasite load in PKDL lesion tissues.

Conclusion

The study found that elevated levels of nTreg cells and IL-10 in PKDL lesions correlate with higher parasite burden.

Supporting Evidence

  • Elevated mRNA levels of nTreg markers were found in pre-treatment PKDL cases compared to controls.
  • Foxp3, CD25, and IL-10 mRNA levels directly correlated with parasite load in lesion tissues.
  • Post-treatment, mRNA levels of nTreg markers and IL-10 were significantly reduced.

Takeaway

This study shows that certain immune cells and a molecule called IL-10 are linked to how much parasite is in the skin of people with a specific disease called PKDL.

Methodology

The study involved ex-vivo mRNA and protein analysis of nTreg markers and IL-10 levels in lesion tissues of PKDL patients.

Participant Demographics

{"age_range":"19–52","mean_age":27.72,"sex_ratio":"22 males, 3 females"}

Statistical Information

P-Value

0.0009

Statistical Significance

p<0.0001

Digital Object Identifier (DOI)

10.1371/journal.pntd.0001171

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