Comparative Analysis of Mycobacterium avium subsp. paratuberculosis Isolates
Author Information
Author(s): Iker Sevilla, Li Lingling, Amonsin Alongkorn, Garrido Joseba M, Geijo Maria V, Kapur Vivek, Juste Ramón A
Primary Institution: Instituto Vasco de Investigación y Desarrollo Agrario (NEIKER)
Hypothesis
This study aims to assess the usefulness of PCR-based short sequence repeat analysis in epidemiological tracing of paratuberculosis strains.
Conclusion
The combined approach of SSR and PFGE provides the highest level of discrimination for Mycobacterium avium subsp. paratuberculosis strain typing.
Supporting Evidence
- Nineteen different multi-locus SSR types were identified among the 268 isolates.
- SSR type 7_4 was the predominant genotype, accounting for 51.2% of all isolates.
- Both SSR and PFGE methods showed similar discriminatory levels.
- Combination of both typing methods revealed 20 multi-type farms out of the 33 bovine farms studied.
Takeaway
Researchers looked at different types of bacteria that cause a disease in cows, sheep, and goats to see how they can tell them apart better.
Methodology
The study compared 268 isolates using short sequence repeat (SSR) analysis and pulsed-field gel electrophoresis (PFGE) typing methods.
Limitations
The study may be limited by the genetic homogeneity of Mycobacterium avium subsp. paratuberculosis isolates.
Participant Demographics
Isolates were collected from cattle, sheep, goats, bison, deer, and wild boar.
Digital Object Identifier (DOI)
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