Activation of ATP-ubiquitin-dependent proteolysis in skeletal muscle in vivo and murine myoblasts in vitro by a proteolysis-inducing factor (PIF)
2001
How a Tumor Product Affects Muscle Breakdown
Sample size: 7
publication
Evidence: moderate
Author Information
Author(s): Lorite M J, Smith H J, Arnold J A, Morris A, Thompson M G, Tisdale M J
Primary Institution: Pharmaceutical Sciences Research Institute, Aston University
Hypothesis
The study examines the mechanism of catabolism of skeletal muscle by a tumour product, proteolysis-inducing factor (PIF).
Conclusion
PIF stimulates the proteasome pathway in muscle cells, contributing to protein breakdown in cancer cachexia.
Supporting Evidence
- Intravenous administration of PIF to normal mice resulted in a rapid decrease in body weight.
- Increased mRNA levels for ubiquitin and proteasome components were observed in muscle tissue.
- Protein degradation was enhanced in C2C12 myoblasts after PIF addition.
Takeaway
This study found that a substance from tumors can make muscles break down faster, which is bad for people with cancer.
Methodology
Intravenous administration of PIF to mice and analysis of muscle tissue and myoblasts.
Participant Demographics
Normal mice and C2C12 myoblasts.
Digital Object Identifier (DOI)
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