p53 Mutations in Urinary Bladder Cancer
Author Information
Author(s): Berggren P, Steineck G, Adolfsson J, Hansson J, Jansson O, Larsson P, Sandstedt B, Wijkström H, Hemminki K
Primary Institution: Department of Biosciences at Novum, Karolinska Institute
Hypothesis
The study investigates the presence of p53 mutations in patients with urinary bladder neoplasms.
Conclusion
The study found that p53 mutations are more common in highly malignant urinary bladder neoplasms compared to lowly malignant ones.
Supporting Evidence
- The study found p53 mutations in 26 (14%) of the 189 patients.
- Only one mutation was found in a lowly malignant urinary bladder neoplasm.
- 30% of the samples had loss of heterozygosity for p53 markers.
- 31 samples showed loss of heterozygosity but were not mutated.
Takeaway
The researchers looked at 189 patients with bladder cancer and found that only a small number had mutations in a specific gene called p53, which is important for cancer development.
Methodology
The study screened for mutations in exons 5–8 of the p53 gene using PCR and F-SSCP methods.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website