Role of nadA and norB Genes in Aflatoxin G1 Formation
Author Information
Author(s): Ehrlich Kenneth C., Scharfenstein Leslie L. Jr., Montalbano Beverly G., Chang Perng-Kuang
Primary Institution: Southern Regional Research Center
Hypothesis
Are the genes nadA and norB involved in the formation of Aflatoxin G1?
Conclusion
The study provides evidence that the biosynthesis of Aflatoxin G1 involves the reduction by NadA and oxidation by NorB.
Supporting Evidence
- The study shows that nadA mutants accumulate a 386 Da compound, indicating its role in Aflatoxin G1 biosynthesis.
- NorB is suggested to catalyze the final oxidation step in the formation of Aflatoxin G1.
- Previous studies indicated that certain Aspergillus species require specific enzymes for Aflatoxin production.
Takeaway
This study found that two specific genes in a fungus help it make a harmful substance called Aflatoxin G1, which can be dangerous in food.
Methodology
Gene disruption techniques were used to create mutants of Aspergillus parasiticus, followed by TLC and LC/MS analyses of the metabolites produced.
Limitations
The study does not explore the functional implications of the differences in nadA sequences between A. parasiticus and A. flavus.
Digital Object Identifier (DOI)
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