Female-biased expression of long non-coding RNAs in mouse brain
Author Information
Author(s): Reinius Björn, Shi Chengxi, Hengshuo Liu, Sandhu Kuljeet Singh, Radomska Katarzyna J, Rosen Glenn D, Lu Lu, Kullander Klas, Williams Robert W, Jazin Elena
Primary Institution: Uppsala University
Hypothesis
Is sex-biased gene expression in the mammalian brain globally or locally regulated?
Conclusion
The study found that sex-biased gene expression varies by brain region in mice, with many female-biased genes located on the X-chromosome escaping X-inactivation.
Supporting Evidence
- 173 sex-biased genes were identified in the striatum.
- Female-biased genes were found to be clustered on the X-chromosome.
- RNA-DNA FISH experiments confirmed biallelic expression of certain genes.
- Three out of four identified lncRNAs are positioned close to coding genes that escape X-inactivation.
Takeaway
This study looked at how genes behave differently in male and female mouse brains, finding that some genes that are more active in females are located near other important genes on the X-chromosome.
Methodology
Genome-wide microarray analysis of RNA expression in various brain regions of male and female mice.
Limitations
The study used two different microarray platforms, which may affect direct comparisons of gene expression data.
Participant Demographics
Adult male and female mice.
Statistical Information
P-Value
p ≤ 0.05
Statistical Significance
p ≤ 0.05
Digital Object Identifier (DOI)
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