Effects of Lacticaseibacillus paracasei P4 on Fat Cell Metabolism
Author Information
Author(s): Grigorova Natalia, Ivanova Zhenya, Petrova Valeria, Vachkova Ekaterina, Beev Georgi
Primary Institution: Trakia University
Hypothesis
The study investigates the metabolic effects of newly isolated Lacticaseibacillus paracasei strains on adipocyte metabolism.
Conclusion
Lacticaseibacillus paracasei P4 enhances glucose uptake and promotes lipid metabolism in fat cells without increasing fat storage.
Supporting Evidence
- Both L. paracasei strains increased glucose uptake by over 30%.
- P4 significantly upregulated adiponectin and beta-oxidation-related genes.
- M2.1 increased intracellular lipid accumulation, while P4 improved lipid turnover.
Takeaway
This study shows that a specific bacteria can help fat cells use sugar better and manage fat without storing too much.
Methodology
Mature 3T3-L1 adipocytes were treated with cell-free supernatants from L. paracasei strains for 24 hours, followed by assessments of glucose uptake, lipid accumulation, and gene expression.
Limitations
The study is based on in vitro experiments and uses gene expression as a proxy for metabolic changes, which may not always reflect functional activity.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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