Shc Protein's Role in Dendritic Cell Maturation
Author Information
Author(s): Chen Kuang-Den, Hsu Li-Wen, Goto Shigeru, Yeh Chin-Wei, Nakano Toshiaki, Lai Chia-Yun, Chang Yen-Chen, Hou Chiung-Hui, Wang Chih-Chi, Cheng Yu-Fan, Chiu King-Wah, Lin Chih-Che, Chen Chao-Long
Primary Institution: Center for Translational Research in Biomedical Sciences, Liver Transplantation Program, Departments of Surgery, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan
Hypothesis
The study aims to evaluate the immune-regulatory role of Shc in rat bone marrow-derived dendritic cells during maturation triggered by LPS.
Conclusion
Shc blockade in LPS-primed dendritic cells leads to the development of tolerogenic dendritic cells via Src-dependent STAT3 activation.
Supporting Evidence
- Shc proteins were induced by LPS in dendritic cells.
- Blocking Shc led to decreased IL-6 and IL-12 production.
- Shc silencing enhanced IL-10 production and CD80 expression.
Takeaway
The Shc protein helps control how dendritic cells mature and respond to infections, and blocking it can change their behavior to be more tolerant.
Methodology
Bone marrow-derived dendritic cells from Lewis rats were treated with LPS and analyzed for cytokine production and surface marker expression.
Participant Demographics
Male Lewis rats, four weeks old.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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